Mac

Tag Antibody 15089

字号+ 作者:果小编 来源:原创 2018-01-14 07:00 我要评论()

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the foll......

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (Macbook Pro) is the 370 amino acid product of the E.coli mal E gene. Macbook Pro is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The Macbook Pro tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the Macbook Pro domain that allow the synthesis of high levels of Macbook Pro-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the Macbook Pro protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, Macbook Pro fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the Macbook Pro-tag. This antibody recognizes Macbook Pro (Maltose binding protein) TAG in some expression systems.

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